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タイトル: Expression of recombinant dense granule protein 7 of Neospora caninum and evaluation of its diagnostic potential for canine neosporosis
著者: Hara, Olgga A
Liao, Min
Baticados, Waren
Bannai, Hiroshi
Zhang, Guohong
Zhang, Sofa
Lee, Eung-goo
Nishikawa, Yoshifumi
Claveria, Florencia G.
Igarashi, Makoto
Nagasawa, Hideyuki
Xuan, Xuenan
西川, 義文
五十嵐, 郁男
玄, 学南
キーワード: Canine
ELISA
Neospora caninum
NcGRA7
発行日: 2006
出版者: National Research Center for Protozoan Diseases
誌名: The Journal of protozoology research
巻: 16
開始ページ: 34-41
抄録: Neospora caninum infection is an important disease affecting bovine and canine populations worldwide. The dense granule antigen 7 of N. caninum (NcGRA7) is considered to be an immunodominant antigen. In the present study, the gene encoding truncated NcGRA7 (NcGRA7t) lacking an N-terminal signal peptide was expressed in Escherichia coli, and its diagnostic potential in an enzyme-linked immunosorbent assay (ELISA) was evaluated. The ELISA could clearly discriminate between known N. caninum-positive and -negative sera from dogs. Serum samples randomly collected from dogs in Japan and China were examined for the diagnosis of neosporosis using the ELISA. Twenty-three of 135 samples (17.03%) and 9 of 95 (9.47%) samples from Japan and China, respectively, were positive for antibodies to N. caninum. Of the 32 ELISA-positive samples, 29 (90.62%) were confirmed as positive by Western blot analysis with whole tachyzoite antigens. These results indicate that the recombinant NcGRA7t would be a reliable antigen in ELISA for the diagnosis of canine neosporosis.
URI: http://ir.obihiro.ac.jp/dspace/handle/10322/995
権利情報: National Research Center for Protozoan Diseases
出現コレクション:05001学術雑誌論文

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