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タイトル: Babesia gibsoni: An apical membrane antigen-1 homologue and its antibody response in the infected dogs
その他のタイトル: An apical membrane antigen-1 homologue from Babesia
著者: Zhou, Jinlin
Yang, Jun
Zhang, Guohong
Nishikawa, Yoshifumi
Fujisaki, Kozo
Xuan, Xuenan
西川, 義文
玄, 学南
キーワード: Babesia gibsoni
AMA-1
antibody response
発行日: Dec-2006
出版者: ACADEMIC PRESS INC ELSEVIER SCIENCE
誌名: EXPERIMENTAL PARASITOLOGY
巻: 114
号: 4
開始ページ: 329-333
抄録: A cDNA encoding the apical membrane antigen-1 (AMA-1) homologue was obtained by immunoscreening a cDNA expression library prepared from Babesia gibsoni merozoite mRNA. The complete nucleotide sequence of the gene was 2062 bp. Computer analysis suggested that the sequence contains an open reading frame of 1794 bp with a coding capacity of approximately 66 kDa. Based on the homology analysis, this putative protein was designated as B. gibsoni AMA-1 (BgAMA-1). The BgAMA-1 gene was expressed in the Escherichia coli BL21 strain and used as the antigen in Western blotting and the enzyme-linked immunosorbent assay (ELISA). The results indicated that BgAMA-1 was recognized as an immunodominant antigen by the host immune system and that it induced a strong antibody response only in chronic B. gibsoni infection in dogs; however, the antibody response could not be detected in the early infection stage (within 15 days). This phenomenon might be explained by the limited stimulation with the low-abundance protein in the early infection stage. This result shows that BgAMA-1 is a new member of the AMA-1 family and that its immune response is characteristic of canine B. gibsoni infection.
URI: http://ir.obihiro.ac.jp/dspace/handle/10322/815
ISSN: 0014-4894
出現コレクション:05001学術雑誌論文

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