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タイトル: Evaluation of recombinant antigens in combination and single formula for diagnosis of feline toxoplasmosis
著者: Abdelbaset, Abdelbaset Eweda
Alhasan, Hend
Salman, Doaa
Karram, Mohamed Hassan
Rushdi, Mahmoud Abd Ellah
Xuan Xuenan
Igarashi, Makoto
キーワード: ELISA
Recombinant proteins
Serodiagnosis
Toxoplasmosis
発行日: 2017
出版者: Elsevier
誌名: Experimental Parasitology
巻: 172
開始ページ: 1
終了ページ: 4
抄録: Cats are the only definitive hosts of Toxoplasma gondii and constitute an essential source of infection to all warm blooded animals and humans. Diagnosis of T gondii infection in cats is fundamental for proper management and control of infection in humans and animals. In the current study, we have evaluated the diagnostic performance of tachyzoite lysate antigen (TLA) and different T. gondii recombinant antigens including surface antigen 2 (SAG2), dense granule proteins 2, 6, 7, 15 (GRA2, GRA6, GRA7, GRA15) and microneme 10 protein (MIC10) in immunoglobulin G enzyme linked-immunosorbent assay (IgG ELISA) using cat serum samples, with reference to latex agglutination test (LAT). Remarkably, TLA showed better performance than other recombinant antigens in IgG ELISAs as compared to LAT, with concordance and Kappa values of 94.27% and 0.93, respectively. Furthermore, to improve the reactivity of the recombinant antigens, we have developed IgG ELISAs using different combinations with these recombinant antigens. Strikingly, a combination of SAG2 and GRAs has relatively similar performance as TLA evidenced by concordance and Kappa values of 94.27% and 0.81, respectively. The developed ELISA with a combination of recombinant antigens can be used as a promising diagnostic tool for routine testing of T. gondii infection and mass screening in cats. The major advantages of this assay are the high sensitivity and specificity, lower cost, safer production and easiness of standardization in various laboratories worldwide. (C) 2016 The Authors. Published by Elsevier Inc.
URI: http://ir.obihiro.ac.jp/dspace/handle/10322/4574
DOI: info:doi/10.1016/j.exppara.2016.11.003
ISSN: 0014-4894
権利情報: This accepted manuscript is licensed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. <http://creativecommons.org/lisenses/by-nc-nd/4.0/>
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