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タイトル: The PCR detection and phylogenetic characterization of Babesia microti in questing ticks in Mongolia
著者: Tuvshintulga, Bumduuren
Sivakumar, Thillaiampalam
Battsetseg, Badgar
Narantsatsaral, Sandag-ochir
Enkhtaivan, Batsaikhan
Battur, Banzragch
Hayashida, Kyoko
Okubo, Kazuhiro
Ishizaki, Takahiro
Inoue, Noboru
Igarashi, Ikuo
Yokoyama, Naoaki
キーワード: 18S rRNA
B. microti
Cox1
Mongolia
Ticks
TufA
発行日: Dec-2015
出版者: Elsevier Ireland
誌名: Parasitology International
巻: 64
号: 6
開始ページ: 527
終了ページ: 532
抄録: Babesia microti is a tick-transmitted zoonotic hemoprotozoan parasite. In the present study, we investigated B. microti infection in questing ticks in Mongolia. A total of 219 questing ticks were collected from three different Mongolian provinces (Bayan-Olgii, Khovsgol, and Selenge). Of these, 63 from Selenge were identified as Ixodes persulcatus, while the remaining 156 (from all three provinces) were identified as Dermacentor nuttalli. When the tick DNA samples were screened using a B. microti-specific nested PCR, 19 (30.2%) of the 63 I. persulcatus ticks were found to be B. microti-positive. The parasite was not detected in D. nuttalli. Subsequently, the 18S rRNA, cox1, and tufA sequences of B. microti were amplified, sequenced, and subjected to phylogenetic analyses. Sequencing analyses showed that the Mongolian 18S rRNA, cox1, and tufA sequences were 99.6-100%, 96.7-97.2%, and 94.7-95.3% homologous, respectively, with B. microti R1 strain US-type sequences from humans. In the phylogenetic analyses, the Mongolian cox1 and tufA sequences were found to be separate lineages, which formed sister-clades to the R1 strain sequences, while all of the Mongolian B. microti 18S rRNA sequences were clustered within US-type clade containing several other sequences of human origin. In conclusion, in addition to reporting the presence of B. microti for the first time in questing ticks in Mongolia, the present study found that Mongolian I. persulcatus ticks were infected with US-type B. microti. These findings warrant large-scale studies to detect and characterize B. microti in ticks, small mammals, and humans. Such studies should provide us with a better understanding of zoonotic Babesia epidemiology in Mongolia. c 2015 Elsevier Ireland Ltd.
URI: http://ir.obihiro.ac.jp/dspace/handle/10322/4554
DOI: info:doi/10.1016/j.parint.2015.07.007
ISSN: 13835769
権利情報: This accepted manuscript is licensed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. <http://creativecommons.org/lisenses/by-nc-nd/4.0/>
出現コレクション:05001学術雑誌論文

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