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タイトル: Ca2+-activated Cl- channel currents in mammary secretory cells from lactating mouse
著者: Kamikawa, Akihiro
Ichii, Osamu
Sakazaki, Junpei
Ishikawa, Toru
キーワード: CaCC
lactation
mammary gland
mammary secretory cell
patch-clamp
発行日: 1-Nov-2016
出版者: American Physiological Society
誌名: American Journal of Physiology-Cell Physiology
巻: 311
号: 5
開始ページ: C808
終了ページ: C819
抄録: The Cl- secretion via Ca2+-activated Cl- channel (CaCC) is critical for fluid secretion in exocrine glands like the salivary gland. Also in the mammary gland, it has been hypothesized that CaCC plays an important role in the secretion of Cl- and aqueous phase of milk. However, there has been no evidence for the functional expression of CaCC in native mammary secretory (MS) cells of lactating animals. We therefore assessed membrane current in MS cells that were freshly isolated from lactating mice using whole cell patch-clamp techniques. In MS cells, we detected CaCC current that exhibited the following characteristics: 1) Ca2+-dependent activation at the concentrations of sub-micromolar range; 2) voltage-dependent activation; 3) slow kinetics for activation and deactivation; 4) outward rectification of the steady-state current; 5) anion permeability in the sequence of I- > NO3- > Br- > Cl- >> glutamate; 6) inhibition by Cl- channel blockers (niflumic acid, DIDS, and CaCCinh-A01). These characteristics of native CaCC current were similar to reported characteristics of heterologously expressed TMEM16A. RT-PCR analyses showed the expression of multiple CaCC channels including TMEM16A, Best1, and Best3 in the mammary glands of lactating mice. Immuno-histochemical staining revealed the localization of TMEM16A protein at the apical membrane of the MS cells. Collectively, our data strongly suggest that MS cells functionally express CaCC, which is at least partly constituted by TMEM16A. The CaCC such as TMEM16A at the apical membrane of the MS cells may influence the quantity and/ or quality of milk.
URI: http://ir.obihiro.ac.jp/dspace/handle/10322/4553
DOI: info:doi/10.1152/ajpcell.00050.2016
ISSN: 0363-6143
出現コレクション:01A01学術雑誌論文

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