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タイトル: Evaluation of novel oocyst wall protein candidates of Toxoplasma gondii
著者: Salman, Doaa
Okuda, Liria Hiromi
Ueno, Akio
Dautu, George
Zhang, Feifei
Igarashi, Makoto
キーワード: T. gondii oocyst wall protein
Toxoplasma gondii
発行日: 2017
出版者: Elsevier Ireland
誌名: Parasitology International
巻: 66
号: 5
開始ページ: 643
終了ページ: 651
抄録: Oocyst stage of Toxoplasma gondii is characterized by a durable wall that confers a strong protection to this protozoan parasite in face of harsh environmental conditions. Thus, it is considered the key for transmission of T. gondii. Analysis of oocyst wall composition is mandatory therefore; the aim of this study was to identify novel T. gondii oocyst wall proteins and test their use in detection of these oocysts in environmental samples. Five candidates of novel T. gondii oocyst wall proteins (TgOWPs) were identified and named TgOWP8 through TgOWP12. Recombinant protein of TgOWP8 was expressed in E. coli using glutathione S-transferase as fusion protein. Polyclonal antibody was produced and validated by indirect immunofluorescence antibody assay (IFA). For detection by IFA, we used different methods for fixation and permeabilization of oocysts to improve the antigen-antibody detection. Specificity to wall of T. gondii oocyst was confirmed and revealed absence of cross reactivity with bradyzoite cyst wall and tachyzoites. Although some TgOWPs were identified previously, our study represents a continuation of molecular investigations of oocyst wall proteins as an essential structure for the longevity and infectivity of this stage and also provided new trial to improve T. gondii oocysts detection. c 2017 The Authors
URI: http://ir.obihiro.ac.jp/dspace/handle/10322/4541
DOI: info:doi/10.1016/j.parint.2017.05.009
ISSN: 13835769
権利情報: This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/BY-NC-ND/4.0/).
出現コレクション:05001学術雑誌論文

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