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タイトル: Multiple infections of Anaplasma platys variants in Philippine dogs
著者: Adrian Patalinghug Ybañez
Rochelle Haidee Daclan Ybañez
Yokoyama, Naoaki
Inokuma, Hisashi
キーワード: Anaplasma platys
Dogs
Philippines
Variants
発行日: 2016
出版者: Veterinary World
誌名: Veterinary World
巻: 9
号: 12
開始ページ: 1456
終了ページ: 1460
抄録: Aim: Anaplasma platys, the causative agent of infectious canine cyclic thrombocytopenia, is a tick-borne pathogen that also has been implicated as potentially zoonotic. To provide molecular evidence on the multiple infections of A. platys variants in Philippine dogs. Materials and Methods: DNA fragments of A. platys from infected dogs in the Philippines were molecularly characterized. For screening, 25 dogs suspected to have canine anaplasmosis were tested using a 16S rRNA-based nested polymerase chain reaction (PCR). Infection was confirmed by sequencing of positive amplicons. Second round PCR targeting a longer 16S rRNA fragment was subsequently performed on the first round PCR amplicons of the positive samples. Further characterization using the heat-shock operon (groEL) gene was also performed on the A. platys-positive samples. Results: 10 16S rRNA sequences were obtained and found 99.6-100% identical to each other and 99.6-99.7% identical to the closest registered A. platys sequences. On the other hand, 36 groEL clone sequences were obtained and found to be 85.1-99.8% identical with each other and 85.0-88.9% identical to the closest previously registered A. platys sequences. Four dogs were found coinfected with 2-3 groEL variant sequences. Phylogenetic analyses suggest that the detected A. platys in the Philippines may represent unique variants. Conclusion: A. platys variants were detected in Philippine dogs. Coinfection of different A. platys variants in dogs was also demonstrated. The present study may indicate the potential genetic diversity of A. platys in the country. c Ybanez, et al.
URI: http://ir.obihiro.ac.jp/dspace/handle/10322/4537
ISSN: 09728988
権利情報: This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/).
出現コレクション:01A01学術雑誌論文

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