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タイトル: 凍結乾燥によるマウス精子の保存
その他のタイトル: Freeze-drying Spermatozoa in Mice
著者: 鈴木, 宏志
川瀬, 洋介
キーワード: Mice
Freese-drying
Spermatozoa
マウス
凍結乾燥
精子
発行日: 2008
出版者: 低温生物工学会
誌名: 低温生物工学会誌
巻: 54
号: 1
開始ページ: 21
終了ページ: 27
抄録: Cryopreservation of mouse spermatozoa has been widely applied for maintenance of genetically modified mouse strains. Although cryopreservation of mouse spermatozoa is simpler, less time-consuming, and less costly than of embryos for preservation of the genetic materials, maintenance of cryopreserved spermatozoa still has a high running cost because of the need for a constant supply of liquid nitrogen. In 1998, it has been reported that freeze-dried mouse spermatozoa are capable of participating in normal embryonic development after injection into oocytes, and so they have attracted a great deal of attention as storable gene resources. However, attempts of freeze-dry spermatozoa are not new. Successful conception and full-term development using freeze-dried spermatozoa were reported in the cow and rabbit prior to 1960. Unfortunately, these preliminary results have not been confirmed. Spermatozoa become defective in motility after freeze-drying and are unable to fertilize eggs both in vivo and in vitro. Since freeze-drying of mammalian spermatozoa was demonstrated without loss of genetic or reproductive potential, recent investigations of freeze-dried sperm have focused on the factors affecting freeze-drying spermatozoa in an effort to store the male gamete at ambient temperature. It is particularly essential to assure long-term preservation for several decades or centuries. Recent findings indicate that the pressure levels at primary drying of freeze-dried spermatozoa, in addition to the components of the suspending solution, appears to be an important factor for long-term preservation of freeze-dried spermatozoa in mice.
記述: セミナー「低温と乾燥による生物保存技術への挑戦~マミーエンジニアリング」3.
URI: http://ir.obihiro.ac.jp/dspace/handle/10322/4510
DOI: info:doi/10.20585/cryobolcryotechnol.54.1_21
ISSN: 13407902
権利情報: 低温生物工学会
出現コレクション:05001学術雑誌論文

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