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タイトル: In vitro and in vivo effects of the phytohormone inhibitor fluridone against Neospora caninum infection
著者: Ybañez, Rochelle Haidee D.
Leesombun, Arpron
Nishimura, Maki
Matsubara, Ryuma
Kojima, Mikiko
Sakakibara, Hitoshi
Nagamune, Kisaburo
Nishikawa, Yoshifumi
キーワード: Neospora caninum
Fluridone
Abscisic acid
発行日: Aug-2016
出版者: Elsevier
誌名: Parasitology International
巻: 65
号: 4
開始ページ: 319
終了ページ: 322
抄録: Neospora caninum causes abortion and stillbirth in cattle. Identification of effective drugs against this parasite remains a challenge. Previous studies have suggested that disruption of abscisic acid (ABA)-mediated signaling in apicomplexan parasites such as Toxoplasma gondii offers a new drug target. In this study, the ABA inhibitor, fluridone (FLU), was evaluated for its action against N. caninum. Production of endogenous ABA within N. caninum was confirmed by ultra-performance liquid chromatography-tandem quadruple mass spectrometry. Subsequently, FLU treatment efficacy was assessed using in vitro. Results revealed that FLU inhibited the growth of N. caninum and T. gondii in vitro (IC50 143.1 +/- 43.96 mu M and 330.6 +/- 5238 mu M, respectively). However, FLU did not affect parasite replication at 24 h post-infection, but inhibited egress of N. caninum thereafter. To evaluate the effect of FLU in vivo, N. caninum-infected mice were treated with FLU for 15 days. FLU treatment appeared to ameliorate acute neosporosis induced by lethal parasite challenge. Together, our data shows that ABA might control egress in N. caninum. Therefore, FLU has potential as a candidate drug for the treatment of acute neosporosis.
URI: http://ir.obihiro.ac.jp/dspace/handle/10322/4491
DOI: info:doi/10.1016/j.parint.2016.03.009
ISSN: 1383-5769
権利情報: This accepted manuscript is licensed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. <http://creativecommons.org/lisenses/by-nc-nd/4.0/>
出現コレクション:05001学術雑誌論文

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PARASITOLOGY INTERNATIONAL, 65(4), 319-322, 2016.pdf1.27 MBAdobe PDF見る/開く

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