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タイトル: Development of genome-wide PCR-based markers from insertion, deletion and single nucleotide polymorphisms for closely related Japanese rice cultivars and identification of QTLs for the appearance of cooked rice and polished rice
著者: Kinoshita, Noriko
Takano, Sho
Shimoda, Naomi
Takamure, Itsuro
Sato, Takashi
Kato, Kiyoaki
キーワード: glossiness
whiteness
InDel marker
CAPS marker
dCAPS marker
発行日: Dec-2016
出版者: Japanese Society of Breeding
誌名: Breeding Science
巻: 66
号: 5
開始ページ: 742
終了ページ: 751
抄録: Appearance of rice grain is an important property, affecting its acceptance by consumers. Moreover, appearance is a complex characteristic involving many components, including glossiness and whiteness. The genetic bases for the glossiness of cooked rice and the whiteness of polished rice (WPR) were determined using 133 recombinant inbred lines (RILs) derived from a cross between two closely related cultivars from Hokkaido, Joiku462, with high glossiness and whiteness, and Yukihikari, an ancestor of Joiku462 with low glossiness and whiteness. Analyses identified 167 genome-wide InDel markers, five cleaved amplified polymorphic sequences (CAPS) and eight derived CAPS markers differentiating the parental lines. The glossiness area (GLA) and glossiness strength (GLS) of cooked rice and WPR were determined for RILs in two locations, Pippu and Sapporo, Hokkaido. Four QTLs were detected. qGLA10 and qGLS9 were detected on chromosomes 10 and 9, respectively, with both being significant at both geographic locations. qWPR1 on chromosome 1 was significant at Pippu, and qWPR4 on chromosome 4 was significant at Sapporo. The Joiku462 alleles at all QTLs increased each trait. The PCR-based markers flanking these four QTLs may be useful for improvement of GLA, GLS and WPR.
URI: http://ir.obihiro.ac.jp/dspace/handle/10322/4475
DOI: info:doi/10.1270/jsbbs.16108
ISSN: 1344-7610
出現コレクション:00F01学術雑誌論文

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