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タイトル: Immunization with Toxoplasma gondii peroxiredoxin 1 induces protective immunity against toxoplasmosis in mice
著者: Fereig, Ragab M.
Kuroda, Yasuhiro
Terkawi, Mohamad Alaa
Mahmoud, Motamed Elsayed
Nishikawa, Yoshifumi
発行日: 27-Apr-2017
出版者: PLOS
誌名: Plos One
巻: 12
号: 4
開始ページ: e0176324
抄録: To develop a vaccine against Toxoplasma gondii, a vaccine antigen with immune-stimulating activity is required. In the present study, we investigated the immunogenicity and prophylactic potential of T. gondii peroxiredoxin 1 (TgPrx1). The TgPrx1 was detected in the ascitic fluid of mice 6 days postinfection, while specific antibody levels were low in the sera of chronically infected mice. Treatment of murine peritoneal macrophages with recombinant TgPrx1 triggered IL-12p40 and IL-6 production, but not IL-10 production. In response to TgPrx1, activation of NF-kappa B and IL-6 production were confirmed in mouse macrophage cell line (RAW 264.7). These results suggest the immune-stimulating potentials of TgPrx1. Immunization of mice with recombinant TgPrx1 stimulated specific antibody production (IgG1 and IgG2c). Moreover, spleen cell proliferation and interferon-gamma production significantly increased in the TgPrx1-sensitized cells from mice immunized with the same antigen. Immunization with TgPrx1 also increased mouse survival and decreased cerebral parasite burden against lethal T. gondii infection. Thus, our results suggest that TgPrx1 efficiently induces humoral and cellular immune responses and is useful as a new vaccine antigen against toxoplasmosis.
URI: http://ir.obihiro.ac.jp/dspace/handle/10322/4462
DOI: info:doi/10.1371/journal.pone.0176324
ISSN: 1932-6203
権利情報: This is an open access article licensed under the terms of the Creative Commons Attribution (by) License. <http://creativecommons.org/lisenses/by/4.0/>
出現コレクション:05001学術雑誌論文

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