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タイトル: Sperm Binding to Oviduct Epithelial Cells Enhances TGFB1 and IL10 Expressions in Epithelial Cells as Well as Neutrophils In Vitro: Prostaglandin E-2 As a Main Regulator of Anti-Inflammatory Response in the Bovine Oviduct
著者: Yousef, Mohamed Samy
Marey, Mohamed Ali
Hambruch, Nina
Hayakawa, Hiroyuki
Shimizu, Takashi
Hussien, Hassan Ali
Abdel-Razek, Abdel-Razek Khalifa
Pfarrer, Christiane
Miyamoto, Akio
発行日: 23-Sep-2016
出版者: PLOS
誌名: PLOS ONE
巻: 11
号: 9
開始ページ: e0162309
抄録: Sperm are allogenic to the female genital tract; however, oviducts provide optimal conditions for survival and capacitation of these non-self cells until fertilization. Recently, we showed that oviduct-conditioned media and prostaglandin E-2 (PGE(2)) suppress sperm phagocytosis by polymorphonuclear neutrophils (PMNs) under physiological conditions. We hypothesized that sperm binding to bovine oviduct epithelial cells (BOECs) could change the local innate immunity via PGE2. As the first step to obtain basic information, sub-confluent BOEC monolayers were co-cultured with swim-up sperm for 2 h. BOECs with viable bound sperm were cultured for an additional 3, 6, 12, or 24 h. Then, we confirmed the impact of the sperm-BOEC binding on both BOECs and PMN gene expression. Immunohistochemistry revealed that BOECs strongly express TGFB1 and IL10 in the oviduct. Sperm binding to BOECs in culture induced the anti-inflammatory cytokines (TGFB1 and IL10) and PGE(2) production by BOECs. Exogenous PGE(2) in vitro suppressed pro-inflammatory cytokine expression (TNF and IL1B) in BOECs. Moreover, pre-exposure of PMNs to BOEC-conditioned media suppressed the TNF expression, but the BOEC media co-cultured with sperm stimulated PMNs to express TGFB1 and IL10, with increasing PGE(2) secretion. Of note, exogenous PGE(2) led PMNs in vitro to decrease their TNF expression and increase anti-inflammatory cytokines expression. Our findings strongly suggest that BOECs provide an anti-inflammatory environment under physiological conditions and the sperm-BOEC binding further strengthens this milieu thus suppresses PMNs in the bovine oviduct. PGE(2) is likely to drive this stable anti-inflammatory environment in the oviduct.
URI: http://ir.obihiro.ac.jp/dspace/handle/10322/4418
DOI: info:doi/10.1371/journal.pone.0162309
ISSN: 1932-6203
権利情報: This is an open access article licensed under the terms of the Creative Commons Attribution (by) License. <http://creativecommons.org/lisenses/by/4.0/>
出現コレクション:00A01学術雑誌論文

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