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タイトル: Identification and Characterization of a Trypanosoma congolense 46 kDa Protein as a Candidate Serodiagnostic Antigen
著者: ZHOU, Mo
SUGANUMA, Keisuke
RUTTAYAPORN, Ngasaman
NGUYEN, Thu-Thuy
YAMASAKI, Shino
IGARASHI, Ikuo
KAWAZU, Shin-ichiro
SUZUKI, Yasuhiko
INOUE, Noboru
五十嵐, 郁男
井上, 昇
キーワード: diagnosis
ELISA
nagana
TcP46
Trypanosoma
発行日: 2014
出版者: 日本獣医学会
抄録: Trypanosoma congolense is a major livestock pathogen in Africa, causing large economic losses with serious effects on animal health. Reliable serodiagnostic tests are therefore urgently needed to control T. congolense infection. In this study, we have identified one T. congolense protein as a new candidate serodiagnostic antigen. The 46.4 kDa protein (TcP46, Gene ID: TcIL3000.0.25950) is expressed 5.36 times higher in metacyclic forms than epimastigote forms. The complete nucleotide sequences of TcP46 contained an open reading frame of 1,218 bp. Southern blot analysis indicated that at least two copies of the TcP46 gene were tandemly-arranged in the T. congolense genome. The recombinant TcP46 (rTcP46) was expressed in Escherichia coli as a glutathione S-transferase (GST) fusion protein. Western blot analysis and confocal laser scanning microscopy revealed that the native TcP46 protein is expressed in the cytoplasm during all life-cycle stages of the parasite. Moreover, an enzyme-linked immunosorbent assay (ELISA) based on rTcP46 detected the specific antibodies as early as 8 days post-infection from mice experimentally infected with T. congolense. No cross-reactivity was observed in the rTcP46-based ELISA against serum samples from cattle experimentally infected with Babesia bigemina, B. bovis and Anaplasma marginale. These results suggest that rTcP46 could be used as a serodiagnostic antigen for T. congolense infection.
URI: http://ir.obihiro.ac.jp/dspace/handle/10322/3983
ISSN: 1347-7439
0916-7250
出現コレクション:05001学術雑誌論文

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