帯広畜産大学 学術情報リポジトリ >
000研究域 >
00A畜産衛生学研究部門 >
00A01学術雑誌論文 >

このアイテムの引用には次の識別子を使用してください: http://ir.obihiro.ac.jp/dspace/handle/10322/3946

タイトル: An Acute-phase Protein as a Regulator of Sperm Survival in the Bovine Oviduct: Alpha 1-acid-glycoprotein Impairs Neutrophil Phagocytosis of Sperm In Vitro
著者: LIU, Jinghui
A. MAREY, Mohamed
KOWSAR, Rasoul
HAMBRUCH, Nina
SHIMIZU, Takashi
HANEDA, Shingo
MATSUI, Motozumi
SASAKI, Motoki
HAYAKAWA, Hiroyuki
PFARRER, Christiane
MIYAMOTO, Akio
羽田, 真悟
松井, 基純
佐々木, 基樹
宮本, 明夫
キーワード: Alpha 1-acid glycoprotein (AGP)
Bovine
Oviduct
Phagocytosis
Sperm
発行日: 2014
出版者: 日本繁殖生物学会
誌名: Journal of Reproduction and Development
巻: 60
号: 5
開始ページ: 342-348
抄録: We have previously shown that polymorphonuclear neutrophils (PMNs) are present in bovine oviduct fluid under physiological conditions, and that the oviduct provides a microenvironment that protects sperm from phagocytosis by PMNs. Alpha 1-acid glycoprotein (AGP) is a major acute-phase protein produced mainly in the liver that has immunomodulatory functions. AGP mRNA is expressed in extrahepatic organs, such as the lung, kidney, spleen, lymph node, uterus, and ovary. Therefore, in this study, we investigated, 1) the local production of AGP in the bovine oviduct, 2) the effect of AGP on the phagocytic activity of PMNs for sperm and superoxide production and 3) the impact of AGP desialylation on the PMN phagocytosis of sperm. The AGP gene was expressed in cultured bovine oviduct epithelial cells (BOECs) and AGP protein was detected in oviduct fluid. Preexposure of PMNs to AGP at physiological levels impaired PMN phagocytosis for sperm and superoxide generation. The desialylation of AGP eliminated these suppressive effects of AGP on PMN. Scanning electron microscopy revealed that AGP drastically reduced the formation of DNA-based neutrophil extracellular traps (NETs) for sperm entanglement. Additionally, AGP dose-dependently stimulated BOECs to produce prostaglandin E2 (PGE2) which has been shown to partially contribute to the regulation of sperm phagocytosis in the bovine oviduct. AGP and PGE2 at concentrations detected in the oviducts additively suppressed sperm phagocytosis by PMNs. These results provide evidence that locally produced AGP may be involved in protecting sperm from phagocytosis by PMNs in the bovine oviduct.
URI: http://ir.obihiro.ac.jp/dspace/handle/10322/3946
ISSN: 1348-4400
0916-8818
出現コレクション:00A01学術雑誌論文

このアイテムのファイル:

ファイル 記述 サイズフォーマット
miyamoto60 (5).342-348.2014.pdf1.58 MBAdobe PDF見る/開く

このリポジトリに保管されているアイテムはすべて著作権により保護されています。

 

Valid XHTML 1.0! DSpace Software Copyright © 2002-2006 MIT and Hewlett-Packard - ご意見をお寄せください