帯広畜産大学 学術情報リポジトリ >
050原虫病研究センター >
05001学術雑誌論文 >

このアイテムの引用には次の識別子を使用してください: http://ir.obihiro.ac.jp/dspace/handle/10322/3936

タイトル: Seroprevalences of Toxoplasma gondii and Neospora caninum in Pet Rabbits in Japan
著者: SALMAN, Doaa
OOHASHI, Eiji
MOHAMED, Adel Elsayed Ahmed
ABD EL-MOTTELIB, Abd El-Raheem
OKADA, Tadashi
IGARASHI, Makoto
五十嵐, 慎
キーワード: Neospora caninum
rabbit
seroprevalence
Toxoplasma gondii
発行日: 2014
出版者: 日本獣医学会
誌名: Journal of Veterinary Medical Science
巻: 76
号: 6
開始ページ: 855-862
抄録: The potential contamination of Toxoplasma gondii and Neospora caninum oocysts in the human environment is a concern from the public health viewpoint. However, estimation of their seroprevalences in humans cannot be performed in a manner that distinguishes between oocysts and tissue cysts as a source of infection. Rabbits are considered popular pet animals in Japan that can acquire natural infections by the aforementioned parasites only through the ingestion of oocysts. Therefore, this study was conducted to estimate the seroprevalences of T. gondii and N. caninum in pet rabbits in Japan as an indicator of the possible oocyst contamination in the environment surrounding human beings. Serum samples of 337 rabbits were examined by different serological methods. Enzyme-linked immunosorbent assays were performed to measure the titer of IgG and IgM antibodies. Samples revealed to be seropositive by ELISA were further analyzed by a latex agglutination test, Western blotting and an indirect immunofluorescence assay. The rates of seropositivity for T. gondii were 0.89% (3/337) and 0.29% (1/337) in IgG and IgM ELISA, respectively. SAG1 and SAG2 were detected as major antigens by the positive rabbit sera in Western blotting associated with strong staining observed by IFA in T. gondii tachyzoites. Regarding N. caninum, none of the serum samples showed a specific reaction in both Western blotting and the IFA. The results of this study indicate low seroprevalences of toxoplasmosis and neosporosis in pet rabbits in Japan, suggesting low oocyst contamination in the human environment.
URI: http://ir.obihiro.ac.jp/dspace/handle/10322/3936
ISSN: 1347-7439
0916-7250
権利情報: 日本獣医学会
出現コレクション:05001学術雑誌論文

このアイテムのファイル:

ファイル 記述 サイズフォーマット
igarashim76(6).855-862.2014.pdf1.46 MBAdobe PDF見る/開く

このリポジトリに保管されているアイテムはすべて著作権により保護されています。

 

Valid XHTML 1.0! DSpace Software Copyright © 2002-2006 MIT and Hewlett-Packard - ご意見をお寄せください