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タイトル: Specific Molecular Detection and Characterization of Anaplasma marginale in Mongolian Cattle
著者: YBAÑEZ, Adrian Patalinghug
SIVAKUMAR, Thillaiampalam
BATTSETSEG, Badgar
BATTUR, Banzragch
ALTANGEREL, Khukhuu
MATSUMOTO, Kotaro
YOKOYAMA, Naoaki
INOKUMA, Hisashi
松本, 高太郎
横山, 直明
猪熊, 壽
キーワード: Anaplasma marginale
cattle
Mongolia
Msp5
PCR
発行日: 2013
出版者: 日本獣医学会
抄録: Anaplasma marginale is an etiologic agent of bovine anaplasmosis. This study aimed to molecularly detect and characterize A. marginale that is prevalent in Mongolian cattle populations. A highly specific and sensitive nested PCR (nPCR) method based on the Msp5 gene was developed to detect A. marginale (Msp5 nPCR). The method detected A. marginale from the positive DNA samples obtained from different countries, while no amplicons were observed from DNA samples of several other bovine blood pathogens tested. The detection limit of Msp5 nPCR was determined to be 2 copies/μl. The method was tested against field blood DNA samples prepared from 300 Mongolian cattle in 2010. Results indicated a prevalence rate of 8.7% (26 of 300). On the other hand, partial DNA fragments of an Anaplasma sp. closely related to A. ovis (with 95.0% identity) were detected using a different nPCR method based on groEL gene. The phylogenetic analyses based on the Msp5, groEL and 16S rRNA genes demonstrated that A. marginale isolates in Mongolia were not divergent from the isolates distributed in other countries. The present study successfully established a new nPCR assay that can detect A. marginale, and reported the first molecular detection and characterization of A. marginale and an Anaplasma sp. closely related to A. ovis in Mongolian cattle populations.
URI: http://ir.obihiro.ac.jp/dspace/handle/10322/3867
ISSN: 1347-7439
0916-7250
出現コレクション:00B01学術雑誌論文

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