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タイトル: First Molecular Characterization of Anaplasma marginale in Cattle and Rhipicephalus (Boophilus) microplus Ticks in Cebu, Philippines
著者: YBAÑEZ, Adrian Patalinghug
SIVAKUMAR, Thillaiampalam
YBAÑEZ, Rochelle Haidee Daclan
RATILLA, Jowarren Catingan
PEREZ, Zandro Obligado
GABOTERO, Shirleny Reyes
HAKIMI, Hassan
KAWAZU, Shin-ichiro
MATSUMOTO, Kotaro
YOKOYAMA, Naoaki
INOKUMA, Hisashi
河津, 信一郎
松本, 高太郎
横山, 直明
猪熊, 壽
キーワード: Anaplasma marginale
cattle
PCR
Philippines
Rhipicephalus (Boophilus) microplus
発行日: 2013
出版者: 日本獣医学会
抄録: Anaplasma marginale has been detected in the Philippines only by peripheral blood smear examination and serological methods. This study generally aimed to molecularly detect and characterize A. marginale in cattle and ticks in Cebu, Philippines. A total of 12 bovine blood samples and 60 Rhipicephalus (Boophilus) microplus ticks were collected on the Cebu Island in 2011. 16S rRNA-based screening-PCR and DNA sequencing revealed 8 cattle (66.7%) and 8 ticks (13.3%) to be positive for A. marginale, and 1 tick (1.7%) to be positive for A. centrale. Selected positive DNA samples were further characterized based on 16S rRNA (longer sequence), Msp5, Msp1α,gltA and groEL genes for phylogenetic analyses. Sequence identities of partial DNA fragments of A. marginale from the Philippines revealed 99.1–100% (16S rRNA, gltA, groEL and Msp5) and 94.3–97.6% (Msp1α) identities to the closest isolates from other countries. Moreover, sequence analysis of the Msp1α, gene showed 3 variants, including a case of co-infection with 2 variants. Phylogenetic analyses based on Msp1α and Msp5 genes revealed that Philippine A. marginale isolates formed a monophyletic lineage, and were phylogenetically related to Brazilian and Chinese isolates. On the other hand, a highly specific and sensitive nested PCR based on groEL, with a detection limit of 2 copies/PCR, was developed to detect A. marginale in the Philippines. This study reported the first molecular detection and characterization of A. marginale in cattle and R. microplus in Cebu, Philippines.
URI: http://ir.obihiro.ac.jp/dspace/handle/10322/3866
ISSN: 1347-7439
0916-7250
出現コレクション:00B01学術雑誌論文

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