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タイトル: Prevalence and Risk Factor Analysis of Bovine Hemoplasma Infection by Direct PCR in Eastern Hokkaido, Japan
著者: TAGAWA, Michihito
YBANEZ, Adrian P.
MATSUMOTO, Kotaro
YOKOYAMA, Naoaki
INOKUMA, Hisashi
松本, 高太郎
横山, 直明
猪熊, 壽
キーワード: bovine hemoplasma
direct PCR
Japan
risk factor
発行日: 2012
出版者: Japanese Society of Veterinary Science
誌名: Journal of Veterinary Medical Science
巻: 74
号: 9
開始ページ: 1171-1176
抄録: The prevalence and risk factors of 2 bovine hemoplasma species—Mycoplasma wenyonii (Mw) and “Candidatus Mycoplasma haemobos” (CMh)—were examined by direct PCR using whole blood samples collected from 343 cattle in Eastern Hokkaido, Japan. The sensitivity of the direct PCR assay was 10-fold higher than the standard PCR assay, detecting 5 copies of bovine hemoplasma DNA per reaction. An epidemiological survey of sampling location, living conditions, age and bovine leukemia virus antibody was performed to assess infection risk factors. The prevalence rates were 38.5% for Mw and 39.1% for CMh, respectively, with an overall prevalence rate of 64.7% for all bovine hemoplasma infections. Significant differences were found in sampling location, living conditions and age. Compared with pastured cattle, farmed cattle were significantly more susceptible to infection with total hemoplasma species (P<0.001), and were particularly susceptible to CMh (P<0.001). Cattle that were 1–3 years of age were more susceptible to infection with hemoplasma compared with other age groups. Bovine leukemia virus infection status was not significantly associated with hemoplasma infections. Blood examination revealed significantly lower RBC, Hb, and PCV levels, and a higher MCV in infected cattle than in noninfected cattle.
URI: http://ir.obihiro.ac.jp/dspace/handle/10322/3838
ISSN: 1347-7439
0916-7250
権利情報: Japanese Society of Veterinary Science
出現コレクション:00B01学術雑誌論文

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