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タイトル: Detection of the New Ehrlichia Species Closely Related to Ehrlichia ewingii from Haemaphysalis longicornis in Yonaguni Island, Okinawa, Japan
著者: MATSUMOTO, Kotaro
TAKEUCHI, Toshihiko
YOKOYAMA, Naoaki
KATAGIRI, Yoshito
OOSHIRO, Mamoru
ZAKIMI, Satoshi
Gaowa
KAWAMORI, Fumihiko
OHASHI, Norio
INOKUMA, Hisashi
松本, 高太郎
横山, 直明
猪熊, 壽
キーワード: 16SrRNA
Ehrlichia
groEL
Haemaphysalis longicornis
発行日: 2011
出版者: Japanese Society of Veterinary Science
誌名: The Journal of Veterinary Medical Science
巻: 73
号: 11
開始ページ: 1485-1488
抄録: We collected a total of 206 Haemaphysalis longicornis ticks by flagging in pastures in Yonaguni Island, Okinawa, Japan, in April 2008. Four of the 206 tick DNA samples tested were positive in a polymerase chain reaction (PCR) screening for the 16SrRNA gene of Anaplasmataceae. Partial sequences of 4 PCR products were identical to each other. Longer sequences of the 16SrRNA gene were successfully determined in 2 of the 4 tick samples, and the obtained 1,392 bp and 1,300 bp sequences revealed high similarity to the 16SrRNA gene sequences of the validated Ehrlichia species, including Ehrlichia ewingii, E. chaffeensis, and E. canis (98.3–98.6%). We also sequenced 1,304 bp of the groEL gene from the 2 tick samples, and found that these had the highest similarity to sequences of E. ewingii (94.0–94.4%) in the validated ehrlichial species. Based on the 16SrRNA and groEL gene sequences, the ehrlichial agents detected in this study were similar to the Ehrlichia species detected in Asia and may compose a new Ehrlichia species with other Ehrlichia species detected in Asia.
URI: http://ir.obihiro.ac.jp/dspace/handle/10322/3581
ISSN: 1347-7439
0916-7250
権利情報: Japanese Society of Veterinary Science
出現コレクション:00B01学術雑誌論文

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