帯広畜産大学 学術情報リポジトリ >
050原虫病研究センター >
05001学術雑誌論文 >

このアイテムの引用には次の識別子を使用してください: http://ir.obihiro.ac.jp/dspace/handle/10322/3027

タイトル: Transportation of Freeze-Dried Mouse Spermatozoa Under Different Preservation Conditions
著者: KAWASE, Yosuke
TACHIBE, Takanori
JISHAGE, Kou-ichi
SUZUKI, Hiroshi
鈴木, 宏志
キーワード: Freeze-dried spermatozoa
Intra cytoplasmic sperm injection (ICSI)
Mouse
Transportation
発行日: 2007
出版者: 日本繁殖生物学会
抄録: Freeze-dried mouse spermatozoa can be used for normal embryonic development after injection into oocytes, thus indicating that freeze-drying is a useful method for the storage and transportation of genetic materials from animals. We recently reported that storage of freeze-dried mouse spermatozoa requires maintenance at temperatures lower than -80 C for long-term preservation and a pressure of 0.37 mbar at primary drying and that these conditions significantly improve the developmental rate to the blastocyst stage. In this study, we examined the influence of transportation and preservation conditions on freeze-dried spermatozoa. Freeze-dried spermatozoa stored for 2 or 2.5 years at 4 or -80 C were transported round trip overland between Shizuoka and Hokkaido prefectures in Japan or by air between Japan and Belgium. The freeze-drying conditions consisted of primary drying at pressures of 0.04, 0.37 and 1.03 mbar and secondary drying at a pressure of 0.001 mbar. Embryos (2-cell stage) from freeze-dried spermatozoa dried at 0.04 mbar and stored at 4 C for 2 years with and without overland transportation did not develop to term. The development rates of embryos from spermatozoa stored at -80 C for up to 2 years and transported overland, by air and without transportation were 8, 1 and 28%, respectively. The development rates of embryos from spermatozoa without transportation were significantly higher than with transportation (P<0.05). These data indicate that freeze-dried spermatozoa stored at -80 C with and without transportation can retain their ability to generate viable offspring after storage for up to 2 years. However, there are limitations to be considered in the transportation of freeze-dried spermatozoa at ambient temperature.
URI: http://ir.obihiro.ac.jp/dspace/handle/10322/3027
ISSN: 1348-4400
0916-8818
出現コレクション:05001学術雑誌論文

このアイテムのファイル:

ファイル 記述 サイズフォーマット
transportation.pdf226.27 kBAdobe PDF見る/開く

このリポジトリに保管されているアイテムはすべて著作権により保護されています。

 

Valid XHTML 1.0! DSpace Software Copyright © 2002-2006 MIT and Hewlett-Packard - ご意見をお寄せください