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タイトル: N-acetylglucosaminyltransferase I Activity of Bovine Oviduct Epithelial Cells: Stimulation by Luteinizing Hormone, Vascular Endothelial Growth Factor and Tumor Necrosis Factor α
著者: NOZAKI, Hirofumi
WIJAYAGUNAWARDANE, Missaka P.B.
KODITUWAKKU, Suranga P.
YOSHIDA, Takashi
NAKAMURA, Tadashi
ARAI, Ikichi
URASHIMA, Tadasu
MIYAMOTO, Akio
中村, 正
浦島, 匡
宮本, 明夫
キーワード: Bovine
Luteinizing hormone (LH)
N-acetylglucosaminyltransferase I
Oviduct
Tumor necrosis factor (TNF) α
Vascular endothelial growth factor (VEGF)
発行日: 2005
出版者: 日本繁殖生物学会
誌名: Journal of Reproduction and Development
巻: 51
号: 2
開始ページ: 229-234
抄録: N-acetylglucosaminyltransferase I (GnT I; EC 2.4.1.101), which catalyzes the first step in the conversion of oligomannose to complex or hybrid N-glycans of glycoproteins, was found in media cultured with bovine oviduct epithelial cells (BOEC) obtained from non-pregnant cows during the follicular phase. Combined treatment with specific hormones increased GnT I release from BOEC. Luteinizing hormone (LH; 10 ng/ml) alone slightly, but together with 17β-estradiol (E2; 1 ng/ml), synergistically increased GnT I activity. Vascular endothelial growth factor (VEGF) and tumor necrosis factor (TNF) α, which have been shown to have their highest activities in the bovine oviduct during the periovulatory period, also increased in GnT I activity. This study provides the first evidence of an increase of GnT I release from BOEC in vitro, and shows that endocrine as well as local factors such as LH, VEGF and TNFα increase this activity. The results suggest that GnT I activity in the bovine oviduct may contribute to the induction of glycosylation and thereby contributing to the provision of the optimal microenvironment for fertilization and early development of the embryos.
URI: http://ir.obihiro.ac.jp/dspace/handle/10322/2978
ISSN: 13484400
09168818
権利情報: 日本繁殖生物学会
出現コレクション:00A01学術雑誌論文

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