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タイトル: Diagnosis of Babesia caballi Infection in Horses by Polymerase Chain Reaction
著者: Xuan, Xuenan
Igarashi, Ikuo
Avarzed, A.
Ikadai, Hiromi
Inoue, Noboru
Nagasawa, Hideyuki
Fujisaki, Kozo
Toyoda, Yutaka
Suzuki, Naoyoshi
Mikami, Takeshi
玄, 学南
五十嵐, 郁男
井上, 昇
キーワード: Babesia caballi
PCR
IFAT
発行日: Apr-1998
出版者: The Research Center for Protozoan Molecular Immunology
誌名: The Journal of Protozoology Research
巻: 8
号: 2
開始ページ: 85-89
抄録: A set of primers were designed according to the published sequence of the gene encoding a rhoptry protein of Babesia caballi, and used to amplify parasite DNA from the blood samples obtained from carrier horses by polymerase chain reaction(PCR)method.The PCR method was sensitive enough to detect parasite DNA from 2.5 μl blood sample with a parasitemia of 0.000001%. The PCR method was compared with fluorescent antibody test(IFAT) in order to evaluate the diagnosis effciency for B. caball infection in horses. Of 142 field samples from Mongolia, 28(20%) and 96(69%)samples were identified positively by PCR and IFAT, respectively. Although the sensitivity of PCR was lower than IFAT, it was noted that the 5 IFAT-negative samples were PCR-positive, suggesting that the combination of PCR method and IFAT for diagnosis of B. caballi infection may be effective in detecting the carrier horses.
URI: http://ir.obihiro.ac.jp/dspace/handle/10322/281
ISSN: 0917-4427
出現コレクション:05001学術雑誌論文

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