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タイトル: Gene silencing of barley P23k involved in secondary wall formation causes abnormal tiller formation and intercalary elongation
著者: Oikawa, Ai
Nagai, Kazuya
Kato, Kiyoaki
Kidou, Shin-ichiro
加藤, 清明
キーワード: Hordeum vulgare
P23k
virus-induced gene silencing
secondary wall formation
(1,3;1,4)-β-D-glucan
tiller
intercalary elongation
発行日: 2009
出版者: 日本育種学会
誌名: Breeding Science
巻: 59
号: 5
開始ページ: 664-670
抄録: P23k is a monocot-unique protein that is highly expressed in barley. Our previous loss-of-function studies in barley leaves indicated that P23k, localized to tissues where cell wall polysaccharides accumulate, might contribute to secondary wall formation in the leaf. However, the P23k loss-of-function analysis was limited to the leaf, which is a vegetative organ. Considering the involvement of P23k in secondary wall formation, a dramatically altered phenotype is expected in the stem of P23k gene-silenced barley, where marked secondary wall deposition occurs during the reproductive growth stage. To test this hypothesis, barley striped mosaic virus-based virus-induced gene silencing of P23k was performed. Abnormal tiller formation and arrested intercalary elongation were observed in P23k-silenced barley. From these results, we speculated that cell wall architecture was altered by P23k gene silencing. Consistent with this idea, we observed a marked decrease in the amount of cell wall polysaccharides stained with calcofluor and down-regulation of the cellulose synthase-like CslF6 gene involved in (1,3;1,4)-β-D-glucan synthesis. Taken together, these results suggest that P23k is possibly involved in determining secondary wall architecture and contributes to tiller formation and intercalary elongation in barley.
URI: http://ir.obihiro.ac.jp/dspace/handle/10322/2692
ISSN: 13473735
13447610
権利情報: 日本育種学会
出現コレクション:00F01学術雑誌論文

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