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このアイテムの引用には次の識別子を使用してください: http://ir.obihiro.ac.jp/dspace/handle/10322/2691

タイトル: Stability of Loop-Mediated Isothermal Amplification (LAMP) Reagents and its Amplification Efficiency on Crude Trypanosome DNA Templates
著者: THEKISOE, Oriel M. M
BAZIE, Raoul S. B
CORONEL-SERVIAN, Andrea M
SUGIMOTO, Chihiro
KAWAZU, Shin-ichiro
INOUE, Noboru
井上, 昇
キーワード: diagnosis
DNA template
LAMP
parasitic disease
Trypanosoma
発行日: Apr-2009
出版者: 日本獣医学会
誌名: The journal of veterinary medical science
巻: 71
号: 4
開始ページ: 471-475
抄録: This study evaluated the stability of LAMP reagents when stored at 25C and 37C, and also assessed its detection efficiency on different DNA template preparations. Accordingly, LAMP using reagents stored at 25C and 37C amplified DNA of in vitro cultured T. b. brucei (GUTat 3.1) from day 1 to day 15 of reagent storage. There were no significant differences (P>0.05) in detection sensitivity of LAMP among the reagents stored at 25C, 37C and –20C (recommended storage temperature). LAMP using the reagents stored at above-mentioned temperatures amplified serially diluted DNAs (genomic DNA extracted by phenol-chloroform method, FTA card and hemolysed blood) of T. b. gambiense (IL2343) with high sensitivity. Reactions were conducted on the reagents stored from 1 day to 30 days. LAMP detection sensitivity was poor when fresh blood as DNA template was added directly into reactive solution. Results of this study demonstrated that LAMP has the potential to be used in field conditions for diagnosis of trypanosome infections without being affected by ambient temperatures of tropical and sub-tropical countries where trypanosomosis is endemic.
URI: http://ir.obihiro.ac.jp/dspace/handle/10322/2691
ISSN: 09167250
出現コレクション:05001学術雑誌論文

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