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タイトル: Loop-mediated isothermal amplification (LAMP) assays for detection of Theileria parva infections targeting the PIM and p150 genes
著者: 河津, 信一郎
Thekisoe, Oriel M.M.
Rambritch, Natasha E.
Nakao, Ryo
Bazie, Raoul S.
Mbati, Peter
Namangala, Boniface
Malele, Imna
Skilton, Robert A.
Jongejan, Frans
Sugimoto, Chihiro
Kawazu, Shin-Ichiro
Inoue, Noboru
井上, 昇
キーワード: Theileria parva
LAMP
Buffalo
Cattle
Diagnosis
発行日: Jan-2010
出版者: Elsevier
抄録: We have developed two loop-mediated isothermal amplification (LAMP) assays for the detection of Theileria parva, the causative agent of East Coast fever (ECF), an economically important cattle disease in eastern, central and southern Africa. These assays target the polymorphic immunodominant molecule (PIM) and p150 LAMP genes. The primer set for each gene target consists of six primers, and each set recognises eight distinct regions on the target gene to give highly specific detection of T. parva. The detection limit of each primer set is 1 fg, which is equivalent to one copy of the PIM and p150 T. parva genes. These PIM and p150 LAMP primer sets amplify DNA of T. parva isolates from cattle and buffalo from different countries including Kenya, South Africa, Tanzania, Rwanda, Uganda and Burundi, indicating their ability to detect T. parva from different countries. With the advantages of simplicity, rapidity and cost effectiveness, these LAMP assays are good candidates for molecular epidemiology studies and for monitoring control programs in ECF-endemic, resource poor countries.
URI: http://ir.obihiro.ac.jp/dspace/handle/10322/2672
ISSN: 00207519
出現コレクション:05001学術雑誌論文

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