帯広畜産大学 学術情報リポジトリ >
050原虫病研究センター >
05001学術雑誌論文 >

このアイテムの引用には次の識別子を使用してください: http://ir.obihiro.ac.jp/dspace/handle/10322/156

タイトル: Rapid Detection of Plastmodium by a New ‘Thick Smear’ Method using Transmission Fluorescence Microscopy : Direct Staining with Acridine Orange
著者: Kawamoto, Fumihiko
キーワード: acridine orange
fluorochrome staining
interference filter
Plasmodium
thick smear
発行日: Oct-1991
出版者: Research Center for Protozoan Molecular Immunology
誌名: The Journal of Protozoology Research
巻: 1
開始ページ: 27-34
抄録: A new staining technique of 'thick smears' was developed tor diagnosis of malaria using transmission fluorescence microscopy. Plasmodium falciparum cultured in vitro was used as a model. The infected blood was mixed 1 : 1 -1 :2 with acridine orange (AO) solution (final concentrations of 50-lOOu.g/ml in 0.01 M Tris-HCI buffer or in PBS, pH 7.0-7.5). Immediately or several minutes later, haemolyzed or non-haemolyzed 'thick smears' were observed directly by fluorescence microscopy using an interference filter specially designed for excitation of AO or commercially available, three (interference-, glass- and triacetyl cellulose film-) types of B-excitation filters with halogen- or daylight-illuminated microscopes. All of these filters were capable of detecting the parasites rapidly in these wet mounts at a magnification of x200 using standard lenses. Among them, the interferencetype filters having higher transmission efficiencies gave the best results. Film filter system, which was the cheapest, could also be used, especially in combination with daylightilluminated microscopes, although it was necessary to be exposed accurately to the direct rays of the sun. These results strongly suggest that transmission fluorescence microscopy using any B-excitation filter in light microscopes may be a useful economic system for rapid diagnosis of malaria.
URI: http://ir.obihiro.ac.jp/dspace/handle/10322/156
ISSN: 0917-4427
出現コレクション:05001学術雑誌論文

このアイテムのファイル:

ファイル 記述 サイズフォーマット
Prot.Vol.1-(4).pdf2.46 MBAdobe PDF見る/開く

このリポジトリに保管されているアイテムはすべて著作権により保護されています。

 

Valid XHTML 1.0! DSpace Software Copyright © 2002-2006 MIT and Hewlett-Packard - ご意見をお寄せください