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タイトル: In Vitro Cultivation of Blood Stream Trypomastigotes of Trypanosoma vivax without Feeder Cell Layers
著者: Hirumi, Hiroyuki
Hirumi, K.
Moloo, S. K.
Shaw, M. K.
キーワード: feeder cell layers
in vitro cultivation
trypomastigotes
発行日: Oct-1991
出版者: Research Center for Protozoan Molecular Immunology
誌名: The Journal of Protozoology Research
巻: 1
開始ページ: 1-12
抄録: Bloodstream trypomastigotes (BSFs) of 2 clones (IL 1392 and IL 3671) of Trypanosoma vivax were cultured without feeder layers in 3 systems. System I: metacyclic-producing cultures of T. vivax IL 1392 were initiated with BSFs derived from infected mice at 27 OC in the presence of feeder layers using TVM-1 medium. Metacyclics harvested were then transferred to flasks containing feeder cells and maintained at 34 OC using TVM-22 medium. Under such conditions, the metacyclics transformed to BSFs. Bloodstream trypomastigotes which were then transferred to new flasks, continued to grow in HMI-162 medium without feeder cells. The maximum density of the BSFs and their shortest population doubling time were 3.5 x l06 / ml and 13.5 h, respectively. System II: metacyclic-producing cultures of T. vivax IL 3671 were initiated with BSFs derived from infected cattle, without feeder layers at / 27 OC using HMI-107 medium. Metacyclics were then transferred to the axenic culture conditions established in System I. They also transformed to BSFs and multiplied in HMI-162 and HMI-163 media. System III: cultures were initiated with proboscides of Glossina morsitans centralis which were infected with T. vivax IL 3671, without feeder layers at 34 OC using HMI-162 medium. Metacyclics emerged from the proboscides, transformed to BSFs and continued to grow in HMI-163 medium. The maximum density and the population doubling time of IL 3671 BSFs in HMI-163 medium were 1.8 x 106 / ml and 16.1 h, respectively.
URI: http://ir.obihiro.ac.jp/dspace/handle/10322/153
ISSN: 0917-4427
出現コレクション:05001学術雑誌論文

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