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タイトル: Protective immunity of blood plasma fraction containing Babesia rodhaini exoantigens in mice
著者: Kamiya, Shiho
Takabatake, Noriyuki
Fujii, Kei
Okamura, Masashi
Iseki, Hiroshi
Yokoyama, Naoaki
Igarashi, Ikuo
キーワード: Babesiosis
Babesia rodhaini
Exoantigen
Protective effect
Mice
発行日: 2005
出版者: National Research Center for Protozoan Diseases
誌名: The Journal of Protozoology Research
巻: 15
開始ページ: 29-40
抄録: Babesia spp. are major hemoprotozoan parasites in animals. Exoantigens, which are soluble antigens released by the parasites during their asexual development, have been successfully applied for the development of a vaccine against babesiosis. In this study, we examined the ability of exoantigens to induce protective immunity in mice against a rodent Babesia parasite, Babesia rodhaini. The BALB/c mice immunized with the blood plasma fraction of B. rodhaini-infected mice (B. rodhaini exoantigens) showed a significant decrease in the lethal rate when challenged with B. rodhaini. Western blot analysis with anti-B. rodhaini exoantigen immune serum specifically detected at least eleven proteins in the lysate of B. rodhaini-infected erythrocytes. Next, a cDNA expression phage library of B. rodhaini was immunoscreened with the anti-B. rodhaini exoantigen immune serum, and three positive cDNA clones were obtained. However, compared to control mice, the mice immunized with each of these recombinant proteins expressed in E. coli or insect cells did not show significantly prolonged survivals after the challenge infection. These findings indicated that B. rodhaini-derived exoantigens contain vaccine candidates inducible of protective immunity in mice, but further investigation is needed to identify the target antigens responsible for the protection.
URI: http://ir.obihiro.ac.jp/dspace/handle/10322/149
ISSN: 0917-4427
出現コレクション:05001学術雑誌論文

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