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タイトル: Development and evaluation of an enzyme-linked immunosorbent assay using recombinant p23 for the detection of antibodies to Cryptosporidium parvum in cattle
著者: Bannai, Hiroshi
Nishikawa, Yoshifumi
Seo, Jin-yong
Nakamura, Chinatsu
Zhang, Sofa
Kimata, Isao
Takashima, Yasuhiro
Li, Junyou
Igarashi, Ikuo
Xuan, Xuenan
西川, 義文
五十嵐, 郁男
玄, 学南
キーワード: Cryptosporidium parvum
p23
ELISA
IFAT
発行日: Apr-2006
出版者: National Research Center for Protozoan Diseases
抄録: An enzyme-linked immunosorbent assay (ELISA) based on the recombinant p23 of Cryptosporidium parvum was established for the detection of antibodies against C. parvum in cattle. The sensitivity and specificity of the ELISA were evaluated with the standard indirect fluorescent antibody test (IFAT) using sporozoites as antigens. Of 77 bovine sera collected from China, 20 (26.0%) were identified as positive by the IFAT. The same samples were tested with the ELISA. The optical densities at 415 nm were compared to the IFAT results and statistically analyzed to designate a provisional cut-off point. As a result, the cut-off point was concluded to be 0.08, which was considered to be the best condition in the light of its sensitivity (80%) and specificity (73.7%). Thus, it was demonstrated that the ELISA could be a useful tool for the diagnosis of C. parvum infection in cattle under field conditions to estimate the magnitude of asymptomatic infection in a cattle population and subsequent risk to humans.
URI: http://ir.obihiro.ac.jp/dspace/handle/10322/142
ISSN: 0917-4427
出現コレクション:05001学術雑誌論文

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